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Since cellulosic particle sizes (external surface areas) influence cellulose accessibility [ 15, 19], they also affect the adsorption of cellulases [ 21, 25] and they are an indication for the maximum cellulase adsorption [ 21].
Maximum cellulase adsorption capacity.
A max cellulase) denotes the maximum cellulase adsorption per g cellulose at equilibrium.
However, in the case of α-cellulose, the increase in maximum cellulase adsorption was disproportionately higher.
However, the pretreatment with swollenin caused a significant increase in maximum cellulase adsorption except for Sigmacell.
Afterwards, the effects of swollenin on cellulose particle size, maximum cellulase adsorption and cellulose crystallinity were quantified.
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This current investigation shows in detail, for the first time, the adsorption of purified cellulases onto α-cellulose, the effect of hydrodynamics on cellulase adsorption and the correlation between the adsorption and the activity of cellulases at different hydrodynamic conditions.
Besides the applied cellulases and substrates, temperature is especially important as it affects cellulase adsorption.
Treatment with LPMO resulted in fibrillation of crystalline cellulose and strongly ( ≥ 2-fold) enhanced the cellulase adsorption.
In this study, the interaction of silica with a cellulase enzyme (CTec2) was studied by addressing the role of silica in cellulase adsorption and cellulose (avicel) hydrolysis.
Optimization of process parameters enhanced cellulase production by 1.7-fold and resulted in maximum cellulase activity of 1.2 FPU/mL in 8 days.
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