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Maximum cell production of 3.57 g/l and yield of 0.18 g/g was observed.
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This may allow for maximum cell survival and production of proteins and other metabolites in these industrially important eukaryotes.
For the production of maximum cell biomass and lipid yield in oleaginous yeasts, medium with an excess of carbon source and limited amount of nitrogen sources has a significant influence (Ratledge 2004).
Both the maximum cell concentration and biotin production varied depending on agitation conditions.
This allowed obtaining a higher maximum cell concentration and increased adenovirus production by minimizing the production of metabolites that can have an inhibitory effect on cell growth.
The analysis of cell-specific mAb production reveals that only AQ outperformed, with a maximum cell-specific mAb production rate 34%% higher than the control (revealing a borderline statistical relevance).
Apparently, uptake mechanisms for members of group B differ from those of group A. Additionally, maximum cell specific growth and mAb production rates were calculated for each supplemented batch culture and compared with a control culture (no dipeptides added).
Isobutanol production and maximum cell density were similar in both strains (Fig. 4a, b).
Thus methods of optimization of the hydrogen production system depend on whether maximum cell activity or maximum H2 output per culture is required.
This altered metabolism led to a 2-fold increase in the maximum cell density and a 25%% increase in recombinant protein production.
Although the maximum specific growth rate remained unchanged, the altered metabolism led to a 2-fold increase in maximum cell density and 33% increase in the integral of viable cell concentration and interferon production yield.
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