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Maximum lipase activity (3.98 U mL−1) and maximum cell mass concentration (5.62 g L−1) were obtained using the optimised medium.
The maintenance coefficient and maximum cell mass yield for this yeast were determined as 5.7 mg glucose/g cell/h and 0.42 g cell/g glucose, respectively, under carbon limitation.
In this study, the maximum cell mass concentration (13.84 g/L) and CHA concentration (48.53 mg/L) were both obtained at 400 rpm (Fig. 5a, b). Figure 5d shows the time courses of CHA production at agitation speed of 400 rpm and the production reached a maximum level of 48.53 mg/L at 336 h.
XMAX: Maximum cell mass produced.
M: at the time of maximum cell mass produced.
QPROT, QLIP, QCARB: Protein, lipid or carbohydrate productivity at maximum cell mass produced.
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QX: Biomass productivity at maximum total cell mass produced.
In order to determine the maximum amount of cell mass that could be efficiently digested by a 13% sodium hypochlorite solution, small-scale extraction experiments were carried out at first.
The maximum expansion in red cell mass varies with the severity of hypoxia – at extreme altitudes, Hb > 210 g/l and Hct > 60% have been reported in acclimatising individuals (Winslow et al, 1984).
It is seen from Figure 6 that maximum conversion was achieved with a cell mass concentration of 200 kg/m3 and decreased thereafter (Additional file 1: Figure 9 of Supporting Information).
As shown in Figure 4a, exponential cell growth was observed after a 15-h lag phase, and the cell mass reached a maximum of 225 mg/L at 54 h.
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