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The negative controls ensure that siRNA transfection has no deleterious effects on cell growth while the positive control provides a reference of maximum cell killing possible and ensures transfection efficiency.
Regardless of the specific conditions tested, maximum cell killing in all experiments was achieved at the lowest tested PRF.
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Maximum cell kill is best achieved by using the optimal (not necessarily the maximum tolerated) dose level as often as feasible considering the impact of the drug on host toxicity.
As observed in the TSP, the majority of the TSQ cells were sensitive to the effects of doxorubicin and cisplatin with a maximum cell kill of 81±10 and 80±12%, respectively.
The authors of one study concluded that a treatment duration of 24 hours, giving a 5-AZA-CdR plasma level of around 2 μM, would be most desirable for maximum cell kill [ 117].
When lowering the extracellular pH from 7.4 to 6.5, tunable functionalized vesicles deliver doxorubicin to cancer cells that increases from 41% to 93% of maximum resulting in cancer cell killing that increases from 23 to 71% of maximum, respectively.
For RIF-1 and CHO cells, maximum levels of carboplatin-induced DNA modification were obtained 24 h after treatment; these levels correlated with cell killing.
Pretreatment with orlistat reduced the cell killing activity of PPD.
Indeed, addition of free targeting peptide reduced cell killing.
In consequence, once tumor cells are arrested and synchronized they can be targeted with a cytotoxic agent, thus maximizing cell kill fraction and minimizing normal cell killing.
Cell adhesion molecules regulate leukocyte trafficking and NK cell killing.
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