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Maximum cell dry weight (238.1 mg/flask) was obtained by day 30.
A central composite design (CCD) was used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry weight in the culture broth with fed batch fermentation in a 5 L fermentor.
A maximum cell dry weight (CDW) of 7.8 g l−1 with a PHB concentration of 5.202 g l−1 was obtained when batch cultivation was conducted at 37°C for 72 h, and the PHB content was up to 66.6% and productivity was 0.072 g l−1 h−1 in 2.0 L fermentor.
The chitinase activity of 1559.2 U/g cell dry weight (36.43 U/mL) and the maximum cell dry weight of 23.3 g/L were reached after incubation of 72 h, which were 39.2-fold and 2.6-fold higher than that of the basic medium, respectively.
In cultures of R. eutropha strains that did not produce polyhydroxyalkanoate or branched-chain alcohols, a maximum cell dry weight of 36 kg m−3 was observed using a fed-batch strategy, when 10 kg m−3 carbon source was fed into culture medium.
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Tanamool et al. [52] reported that maximum 0.920 g/l of cell dry mass and 0.034 g/l PHB using sweet sorghum juice by Ralstonia eutropha with yield and productivity of 0.037 g PHB/g dry cell and 0.0019 g/ l h), respectively.
Under this condition, the maximum cell density observed was 2.74 g dry wt./L after 27 days of cultivation.
The micro-alga shows maximum cell count (×106/ml), optical density (OD680) and dry weight (g L−1) on the twelfth day.
The growth curve indicated that this subisolate exhibited a maximum growth rate of 0.61 g l−1 d−1 (i.e., gram of cell dry weight or CDW per liter per day) and a maximum cell density of 1.98 g l−1 in terms of CDW per liter (Fig. 1a; Table 1).
After that, mcl-PHAs concentration started to increase rapidly, reaching 12.3% CDW at 48 h, when cell dry weight (1.74 g/L) also reached its maximum value.
Under conditions of maximum accumulation, PHAs can constitute up to 90% of the cell dry weight.
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