In this study, the clone K8 was used with an average maximum cell density, i.e., carrying capacity of 300 cells/mL (for more information refer29).
Microfabricated tissues reached the maximum cell density with 16.4× 107 cells/cm3 after 20 days.
The maximum cell density reached in the fermenter during fermentation was 2.4 g/l.
A maximum cell density of 2.4 of was reached in the fermenter during fermentation.
The maximum cell density reached 21.89±0.35 and 20.95±0.01 g DW/l in STR and flasks, respectively.
In bioreactor operated in fed-batch mode, the higher maximum cell density achieved with the PYC2 clone led to a net gain (20%) in final volumetric productivity.
This altered metabolism led to a 2-fold increase in the maximum cell density and a 25%% increase in recombinant protein production.
The response surface plot computed from experimental design was employed to optimize the process operating conditions for maximum cell density, minimum CO residue and maximum acetic acid productivity.
The maximum cell density attained using the optimized Freestyle culture medium was 5.4 × 106 cells/mL in batch mode, almost double of that observed using the unsupplemented medium (2.9 × 106 cells/mL).
Although the maximum specific growth rate remained unchanged, the altered metabolism led to a 2-fold increase in maximum cell density and 33% increase in the integral of viable cell concentration and interferon production yield.
The 17-l FP-ALPBR system was capable of giving reasonable growth characteristics with a maximum cell density of 4.1 × 105 cell ml−1 and specific growth rate of 0.52 day−1 being achieved.
