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Both the maximum cell concentration and biotin production varied depending on agitation conditions.
Here, the cells were at the end of the exponential phase and had attained maximum cell concentration.
The maximum cell concentration and rifamycin B titer were 8.6 g/L and 1260 mg/L, respectively.
In 'normal' fermentation, final glutamate concentration and the maximum cell concentration (OD620) were more than 70 g/L and 50, respectively.
A maximum cell concentration of 0.30 gL−1 was obtained at this sugar concentration as compared to 0.75 gL−1 at 100 gL−1 initial xylose.
This allowed obtaining a higher maximum cell concentration and increased adenovirus production by minimizing the production of metabolites that can have an inhibitory effect on cell growth.
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After the respective maximum, cell concentrations declined and cells entered the death phase.
This represents 1.9-fold improvement over maximum cell concentrations achieved in planar systems, although it is significantly lower than the maximum cell concentrations achieved in xeno-free hPSC cultures performed in microcarrier-based systems (6 × 10 cells/mL) [ 77].
Controlling the dissolved oxygen levels has been found to be critical during hPSC culture on microcarriers in SUBs; 2.5 higher expansion folds and ∼85% improvements in maximum cell concentrations were reported in a hypoxic environment when compared to uncontrolled conditions [ 57].
This result indicted that increase of glucose concentrations enhanced the maximum cell density of the ZS1 culture.
In this study, the maximum cell mass concentration (13.84 g/L) and CHA concentration (48.53 mg/L) were both obtained at 400 rpm (Fig. 5a, b). Figure 5d shows the time courses of CHA production at agitation speed of 400 rpm and the production reached a maximum level of 48.53 mg/L at 336 h.
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maximum cell temperature
maximum cell conversion
maximum cell death
maximum cell loss
maximum cell load
maximum cell speed
maximum chromium concentration
maximum cell viability
maximum cell mass
maximum cell survival
maximum cell area
maximum cell density
maximum blood concentration
maximum cell size
maximum cell retention
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