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After day 3, cells on 100 nm still displayed the maximum cell area.
c After day 1, maximum cell area was displayed by cells on 100 nm which decreased consistently with an increase in the nanopore diameter.
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C6 astrocytes seeded on 50-nm nanodots exhibited maximum cell surface area and cell syncytium, while the cells grown on 100- and 200-nm nanodots showed significant reductions in cell syncytium (Figure 4b).
37 However, striking differences were observed with respect to maximum projected cell area and the time required to reach it.
On the 50-nm nanodot arrays H9c2 showed maximum attachment and proliferation with largest cell area and extended lamellipodia.
The interaction between mCerulean cofilin and eYFP-TG2 gave an average of 15.6% FRET efficiency over the whole cell area (with the maximum possible being ∼30% for this donor acceptor FRET pair) versus the average of the eYFP control interacting with mCerulean cofilin of 0.7% (Fig. 6Aj versus n, quantified in 6B).
This caused the projected cell area to increase, reaching a maximum about 30 minutes after each induced acidification to pH 6.0 (Fig. 6A).
B-E) Morphometric analysis was carried out on images captured at 200x magnification, used to analyse adipocyte cell numbers (B) and cell areas (C), as well as mean (D) and maximum cell widths (E).
A time-dependent decrease in cell area (p<0.0001) and an increase in maximum thickness were seen (p<0.0001).
We count the number of cells in a particular cell area by using the radius of its maximum inscribed circle.
The maximum path loss determines the maximum cell coverage.
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