While differences in minimum and maximum brightness were observed, the concentration of triethylamine had little effect on the repeatability.
You will need a telescope to see this minor planet as its maximum brightness is some 1/4158 of the brightness of the faintest objects that can be seen with the unaided eye.
Thus, even when summing the signal from dozens of pixels, maximum brightness is expected to be in the range of 100 500 photons for standard pixel dwell times (1 4 µs).
If you wake up before the sun comes out, and you can't have sunlight, you can buy a dawn simulator and set it so that the maximum brightness is there when your alarm goes off.
For a given CCD/CMOS sensor, the saturation voltage (corresponding to maximum image brightness) is fixed, leaving us only with the possibility to reduce the noise level in order to increase the dynamic range.
Fluorescent labeling reagents are an essential component of a huge industry built on sensitive fluorescence detection and reagents with close to maximum theoretical brightness are available in many different colors.
For the analysis of this pulse, a sequence of 20 frames from a 30-fps video was stacked, and the maximum brightness values were calculated pixel by pixel by using the software ImageJ (Schneider et al. 2012).
Well, I installed Mountain Lion - which was painless - on my iMac and the first odd things I noticed when it restarted was the screen brightness was at maximum and the Calendar app had been launched.
