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Moreover, Fast-SfS searches for the minimum and the maximum brightness values inside the cropped image.
For the analysis of this pulse, a sequence of 20 frames from a 30-fps video was stacked, and the maximum brightness values were calculated pixel by pixel by using the software ImageJ (Schneider et al. 2012).
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The white circle in Figure 13 shows the location with the maximum brightness value.
According to the considered light reflection model, Figure 4 shows the case in which the currently processed pixel (P x,y)) is characterized by the maximum brightness value in the image.
The formation of higher order oligomers of TLR9 was ruled out because the maximum brightness value observed from the TLR9-GFP was less than thrice that of monomers.
Our all-inorganic full-colour QLEDs show the maximum brightness and efficiency values of 21,600 cd/m2 and 6.52%, respectively, which are record-breaking among the existing all-inorganic QLEDs.
0 dB value corresponded to the maximum brightness that the perimeter can produce (stimulus intensity of 10,000 asb), while 51 dB to the minimum stimulus intensity (0.08 asb).
Then, the brightness values are adjusted according to the following rule and the maximum and minimum intensity values Imax and Imin are computed by the following formula: (3) if x m > 128 ad = 255 − x m else if x m ≤ d min ad = d min else if x m ≤ d max ad = d max else ad = x m, I max = x m + ad, I min = x m − ad.
To estimate how this noise determines the uncertainty of the calculated dF/F0 values and ultimately which SNR can be achieved at a given maximum brightness, we performed a Monte-Carlo analysis: Random Poisson noise was added to the [Ca2+]-dependent fluorescence curves shown in Appendix figure 1A, this was repeated 10,000 times.
To apply it, constant current stress measurements in which the gate-source voltage is continuously adjusted to keep the source-drain current constant at a value that would supply sufficient current to an OLED to reach maximum brightness (Equation 1)—need to be performed to extract the parameters γ, τ, and β.
Considering that the pixels with the maximum brightness (I max ) have an associated angle equal to α H, and the pixels characterized by the minimum value of brightness (I min ) have an associated angle equal to 90° −α H, an αP x,y) value can be assigned to all other pixels in the image by linearly regressing the range [ α H ;90°−α H ] on the pixel brightness range.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com