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The maximum biomass obtained was 33g/L with glycerol and 31 g/L for arabinose, whereas glucose, fructose and sucrose produced 10 g/L biomass (Figure 1b).
The maximum specific growth rate (µ max, day−1) was determined from the different µ values calculated, while the maximum biomass obtained was designated as X max (g/L) (de Morais and Costa 2007).
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Maximum biomass concentration obtained under dark/light cycle (1.30 ± 0.1 g L−1) was higher when compared to continuous illumination (1.12 ± 0.1 g L−1).
The substrate utilization profile was almost similar under different agitation rates but maximum biomass was obtained at higher agitation rate of 700 rpm (Figure 6a, b).
Maximum biomass was obtained on the 16 and 20th day of incubation using wheat straw (5.59 g %) and rice straw (2.1 g %), respectively, and on the 18th day of incubation using wheat bran (5.47 g %) and rice bran (0.92 g %) (Fig. 1b).
The maximum reducing sugar concentration was 200 g/kg which corresponded to 34% per dry biomass obtained at a flow rate of 5 ml/min, temperature of 210 °C during a 45 min treatment.
However, the biomass yield was similar to the biomass obtained by Tang et al. [ 17].
In both strains, maximum biomass density was obtained with glucose, whereas the minimum was achieved with hexadecane as carbon source (Table 1).
The maximum biomass and DHA obtained using optimized (central composite design) media composition after 108 h at 25 °C and 180 rpm were 31 ± 0.2 g L−1 and 6.9 ± 0.05 g L−1 respectively.
The maximum biomass yield was obtained experimentally and in silico under fully aerobic conditions (case 1).
For instance, in continuous cultures, the maximum production of EPA by photoautotrophically grown Nannochloropsis sp. was achieved at dilution rates in the range of 0.004 0.013 h−1 while maximum biomass productivity was obtained at higher dilution rates ranging between 0.011 and 0.017 h−1 (Zou et al. 2000).
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