In a recent study, the optimum temperature of 37°C was observed for the synthesis of biodiesel [ 17], so this shows mesophilic nature of enzyme but the lipase from Bacillus aerius was thermotolerant; therefore maximum biodiesel production was observed at 55°C.
In Table 1, a comparison was given between the oil yield, production, and biodiesel productivity of microalgae with some other biofuel feedstock.
To expand the viability of enzymatic biodiesel production, considerable effort has been directed toward process development in terms of biodiesel productivity, application to wide ranges of contents of water and fatty acids, adding value to glycerol byproducts, and bioreactor design.
A maximum biodiesel yield of 90%(by mass) was obtained.
Biodiesel productivity was also tested using recycled catalyst and a constant yield of biodiesel was obtained for all the catalyst recycle experiments.
In the present work, maximum biodiesel yield and minimum viscosity were assigned as objectives.
The maximum biodiesel yield under the optimized conditions was 99.38 wt.%.
As per the design of experiments, the experimentally obtained maximum biodiesel yield was 95.5% with viscosity 7.14 cSt, whereas the minimum viscosity of biodiesel produced was 5.60 cSt for which the yield of the biodiesel was 93.8%.
The doping process was optimised for maximum biodiesel yield considering four parameters viz.
To ascertain the maximum biodiesel yield from WMSO, a range of transesterification reactions was performed using NaOH and KOH as catalyst.
