Sentence examples for maximum assay concentration from inspiring English sources
The Dundee Drug Discovery Unit (DDU) diversity set of 59,904 compounds was solubilised in DMSO (final maximum assay concentration of 1% (v/v) in all samples including controls) and a HTS performed in singlet at a concentration of 30 μM.
While the effective concentration at 50% of the maximum assay response (EC50) remains one of the most frequently utilized parameters for comparison of concentration response data in biochemical assays, it requires a clearly defined maximum effect level.
Consequently, it is possible that the low background levels and error bars produced in these studies could be further reduced through systematic optimization of the blocking agent, reagent concentrations, and washing conditions, for minimal nonspecific interactions and maximum assay reproducibility.
The typical assay concentration of 0.3% NBD PE gave a good activity of ~60% of the maximum value observed, and again we chose to use this concentration in further experiments.
Analyte assay concentrations were compared.
The focussed kinase library of compounds was solubilised in DMSO with a maximum final assay DMSO concentration of 1%% (v/ v).
CWE was not toxic to cells for 24 and 48 h, but the maximum concentration assayed here (400 μg/ml) caused a 10% decrease in cell viability at the end of 72 h culture period.
Displacement of the fluorescence peak was observed only in Geitlerinema sp. DE2015 from 661 nm (MIF) towards to 670 nm, at maximum Cr-concentration assayed (5 μM fluorescence spectrum).
For binding assays, concentrations producing 50% of the maximum inhibition (IC50) were estimated from Hill models using Prism® 6.02 (GraphPad Software).
Maximum DMSO concentration in the assays was 0.1%, which had no effect on macrophage parasite clearance.
Three out of five tigecycline synergistic concentrations observed in this study with time-kill assays exceed the maximum plasmatic concentration of tigecycline (0.38 mg/L) achievable with a standard dosage [ 41].
