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Maximum amplification efficiency was reached for the allele Desu-DRB*091, which with an efficiency of 2.40 is more than 12 times more efficient than Desu-DRB*028.
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Applied Biosystems guarantee maximum and equivalent amplification efficiency as well as specificity of all TaqMan® Assays-on-Demand Gene Expression Products (Application Note, Applied Biosystems: Amplification Efficiency of TaqMan® Assays-On-Demand™ Gene Expression Products).
QMSP using LNA probe and primers showed an increased amplification efficiency and maximum fluorescent signal.
We used a maximum likelihood approach to estimate the amplification efficiency of each allele (see Methods) and found that there is a substantial variation in the amplification efficiency among alleles.
A guideline to plan future experiments can be derived from the expected maximum number of alleles and the minimum amplification efficiency one could accept (e.g. 10 alleles and a minimum standardised amplification efficiency of 0.3).
We derived a maximum likelihood optimization approach allowing the estimation of the relative amplification efficiency of each allele.
The recombinant RNA competitor was designed to obtain a maximum sequence similarity with the target sequence, and the RT-PCR amplification efficiency of the competitor and target RNA was found to be identical.
The amplification efficiency is enhanced.
Amplification efficiency was calculated by dilution.
Amplification efficiency varied among the other fish species.
PCR amplification efficiency was established by means of calibration curves.
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