Sentence examples for maximum amplicon size from inspiring English sources

The observed fragmentation also resulted in shorter maximum amplicon size in our RT-PCR system.

Primers were designed with the galaxy-pcr-markers pipeline (https://github.com/cfljam/galaxy-pcr-markers) with the modification of producing only one pair of primers, minimum amplicon size of 200 bp and maximum amplicon size of 400 bp.

A size difference of zero can be selected if the user wishes to search for RFLPs or qPCR probes within the products; a size difference of >2 is recommended for detection by capillary electrophoresis; and a size difference of ∼10% of the maximum amplicon size is recommend for detection by agarose gel electrophoresis.

The filtered reads were assessed with Primer3 (Rozen and Skaletsky, 2000) to identify primers using default program settings with slight modifications: melting temperature = 54°C minimum, 58°C optimum, and 62°C maximum; amplicon size = 100 bp minimum, 200 bp maximum; and primer length = 17 bp minimum, 19 bp optimum, and 25 bp maximum.

Data were input from paired "_sequence.txt" files (fastq format with Illumina-specific Phred+64 ASCII score encoding) with a maximum amplicon size of 400nt and minimum identity of 90% for mapping to contigs specified with the flag "-fpp [read file 1] [read file 2] 400 90".

Paired-end data were included from paired "_sequence.txt" files (fastq format with Illumina-specific Phred+64 ASCII score encoding) with a maximum amplicon size of 400nt and minimum identity of 97% for mapping to contigs specified with the flag "-fpp [read file 1] [read file 2] 400 97".

To determine the maximum PCR amplicon size achievable with bisulfite converted DNA from the Qiagen and Epigentek kits, eight primer sets were tested that ranged in amplicon length from 655 4027 bp.

As a matter of fact, we allowed for a wide range of GC content (corresponding to melting temperatures of 57 to 65 °C) but strongly constrained amplicon size differences to a maximum of 200 bp (but actual size differences were much less for most amplicons, see Additional file 2 : Figure S1).

Primer design was carried out using BatchPrimer3 with an optimal GC content of 50%, a maximum melting temperature difference of 3°C, variable amplicon size (to allow multiplexing), and all other parameters set to default values.

Amplicon size was limited to a maximum of 90 bp.

This parameter is of interest for two reasons: (i) short introns are less likely to be polymorphic than longer ones, and (ii) using standard polymerases the amplicon size is limited to a maximum of ~3 kb.