Sentence examples for maximum amplicon lengths from inspiring English sources

The deduced minimum and maximum amplicon lengths were 71 bp (n = 1068) and 94 bp.

Whereas amplicons up to 800 nt can be routinely amplified from samples fixed over night, the maximum amplicon length for samples fixed for 72 hours in our PCR system was around 400 600 nt in most cases (Fig. 3).

The primers used amplified an average of 6,2 Kbp, with a minimum and maximum amplicon length of 3,5 and 9,0 Kbp, respectively (Additional file 1: Table S1).

The filtered reads were assessed with Primer3 (Rozen and Skaletsky, 2000) to identify primers using default program settings with slight modifications: melting temperature = 54°C minimum, 58°C optimum, and 62°C maximum; amplicon size = 100 bp minimum, 200 bp maximum; and primer length = 17 bp minimum, 19 bp optimum, and 25 bp maximum.

Figure 4 Effect of CdTe QD on different amplicon lengths.

PCR primers were designed with the aim to get amplicon lengths of < 200 bp.

Figure 2 Following RT of RNA-derived from human plasma, PCR was performed for increasing amplicon lengths of GAPDH.

The PCR multiplexes amplify short amplicon lengths which makes the assay sensitive to the analysis of degraded DNA.

One amplicon length (representing a single bacterial species) was elevated in most, but not all, MFD-susceptible (S-, M-, or SM-responsive) cows relative to milk fat-nonresponsive cows, whereas 2 amplicon lengths displayed reduced abundance under MFD conditions.

The amplicon lengths were all about 120 bp.

Primer sequences and amplicon lengths are listed in Table S5.