Sentence examples for maximum amplicon length from inspiring English sources

Whereas amplicons up to 800 nt can be routinely amplified from samples fixed over night, the maximum amplicon length for samples fixed for 72 hours in our PCR system was around 400 600 nt in most cases (Fig. 3).

The primers used amplified an average of 6,2 Kbp, with a minimum and maximum amplicon length of 3,5 and 9,0 Kbp, respectively (Additional file 1: Table S1).

The deduced minimum and maximum amplicon lengths were 71 bp (n = 1068) and 94 bp.

Since FFPE tissue can be highly fragmented and of poor quality, design parameters restricted amplicon length to a maximum of 200 nt.

We did not observe a significant correlation between the microsatellite amplicon length (measured as maximum allele size) and error rates (the number of drop outs and false alleles; Figure  3).

Primers were developed using Primer Express version 1.5 (Applied Biosystems Inc., Foster City, USA), using the following parameters: Minimum Tm: 59 60°C, Maximum Tm difference between primers: 1°, Oligo length: 20 25 bp, Amplicon length: 90 120 bp.

The filtered reads were assessed with Primer3 (Rozen and Skaletsky, 2000) to identify primers using default program settings with slight modifications: melting temperature = 54°C minimum, 58°C optimum, and 62°C maximum; amplicon size = 100 bp minimum, 200 bp maximum; and primer length = 17 bp minimum, 19 bp optimum, and 25 bp maximum.

The detection efficiency is enhanced by increasing the ratio of the size difference to amplicon length.

Chloroplast haplotype network using sequence information and PCR amplicon length based on cpSSRs.

The impact of amplicon length and G/C content on HRM scores was also evaluated.

The impact of mutations on HRM scores was influenced by amplicon length and the position of the mutation within the amplicon.