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The maximum alignment depth observed was 344 ESTs in one contig.
Using DNP Trapper, the reads could be divided into two large groups of roughly the same size (group 1: 670 reads with maximum alignment depth 210, indicating approximately 30 copies; group 2: 560 reads, depth 205, around 30 copies) based on their DNP content.
From the maximum alignment depth in each group (470 and 400 respectively), the copy number was estimated to be 70 copies in group 1 and 60 in group 2. Six DNPs in the coding regions were found in coding positions, one of which was present in 160 reads or around 20 copies in one of the groups.
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Hence, we prefer the second alignment as it creates the smallest visual distortion via maximum alignment.
The multiple alignments were analyzed for alignment depth.
Of the alignments created, 4 171 (18%) showed an average alignment depth of 100 or more.
We set a maximum alignment score of 125.
Alignment algorithms find optimum alignments and maximum alignment scores S of two or more sequences for a given scoring system.
Of the 4 171 alignments with an average alignment depth over 100 that were searched for cassette boundaries, 3 788 (91%) yielded estimates.
The table lists the 78 annotated genes with an average alignment depth of 100 or higher.
The sequences with alignment depth of coverage more than 1 were excluded in downstream analysis.
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