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The analysis of statistical parameters during the expression time showed that the velocity at which the enzyme was produced affected cell growth and maximum activity, with a higher speed leading to lower expression and cell growth.
The optimum temperature for the hydroformylation reaction was found to be 240°C, since at this condition one was able to achieve the combination of maximum activity with acceptable selectivity.
Lignin peroxidase from P. chrysosporium showed maximum activity with ferrocyanide followed by N,N-dimethyl-1,4-phenylenediamine, anisyl alcohol, veratryl alcohol and guaiacol (Glumoff et al. 1990).
The compounds (4l) and (4u) exhibited a significant inhibitory activity against A. niger and F. oxysporum with MIC 25 50 μg/mL, whereas, the compounds with floro (4f) and dichloro (4d) also exhibited maximum activity with MIC > 25.
While considering phytopathogens, chloroform extract showed maximum activity with MIC 32 μg/ml for Erwinia sp. and X. campestris.
DMSO impregnated disc was used as negative control whereas standard drug clotrimazole exhibited maximum activity with 30 ± 1.54 mm zone.
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Maximum activities (with ABTS, pH 4.5) were observed at 60°C and 50°C for L Sc and L Tv, respectively (Figure 3a), which is 10°C lower than optimal temperatures reported in other studies (70°C and 60°C respectively) (Arias et al. 2003; Rancaño et al. 2003).
The results also showed that D3A,V266E could be inhibited by XIAP only up to ~ 70% of its maximum activity, compared with ~ 97% for WT (Table 1).
The activity of ArAT from Escherichia coli toward l-aspartate reached 65%% of the maximum activity determined with l-tyrosine (Powell and Morrison 1978), and activity of ArAT from Brevibacterium linens toward l-aspartate reached 50%% of the maximum activity determined with l-phenylalanine (Lee and Desmazeaud 1985).
All prostates were magnetically active, with maximum activity measured between 4,143 and >9,999 units.
This ion had a hyperactivating effect on endoglucanase, with maximum activity of 4.34 U·mL−1 observed with Mn2+ 8 mM which corresponded to an increase of 143% in endoglucanase activity in relation to when no Mn2+ was added.
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