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Both laccase preparations were still active at 10°C, showing 13 and 44% of their maximum activity for L Sc and L Tv, respectively.
This maximum activity for pH = 9 can be related to the effects of the electrode charge and/or water structure in the ORR.
The resultant twelve pairs of stereoisomers at the dioxane C-2 were tested for antiproliferative effect finding the maximum activity for derivatives with methyleneoxy linker between benzodioxane and 2′-methylbiphenyl.
On the other hand, the NH catalysts were more active than the HE catalysts for C N hydrogenolysis and the 973 K-nitrided catalyst showed a maximum activity for C N hydrogenolysis.
Reaction curves were normalized fixing the maximum activity for each protein to 100%.
Hepatic SAM concentration is ∼60 µM and methyl-imbalanced diet typically causes a 1.5 to 3 fold drop in SAM levels [3], [14], which would be still high enough to allow near maximum activity for most methyltransferases with low Km for SAM but would significantly inhibit those enzymes with high Km values.
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Briefly, pH around 7, a final NaCl concentration around 75 mM, and MnCl2 final concentration above 5 mM were necessary to achieve maximum activities for all genotypes (Fig. 4).
We found that the maximum activities for all genes appeared 72 hours after induction.
In this pH range, cellulosomes and CglT appeared to exhibit maximum activities for the substrate.
Maximum activities for the recombinant AmyA and GlaA in S. cerevisiae Y294 were observed at pH 4.0 and pH 4.5, respectively, with significant activity detected for both enzymes at pH 3 to 5.
Unexpectedly, we discover the nucleosome acidic patch(3) as necessary for maximum activity of all ISWI remodelers evaluated.
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