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The maximum activity detected for pH optimum and temperature optimum were defined as 100%.
The maximum activity detected by this method was at pH 5.5 (see Additional file 2).
Similar(58)
The enzyme was stable in the alkaline pH range between 7.0 and 9.0, in which the maximum activity was detected at around pH 8.0 (Figure 3b).
Maximum activity was detected for both enzymes at pH 8.0 and at the temperature of 30°C.
Acetone extract having well to moderate antimicrobial activity and maximum activity was detected in S. epidermidis (18 mm) followed by S. pneumoniae (14 mm).
The chitinolytic activity of the purified CvChi45 was detected over a wide pH range (3.0 to 9.0), but there was no activity detected at pH 2.0 and 10.0; the maximum relative activity was recorded at pH 5.0, with significant hydrolysis of colloidal chitin also detected at pH 6.0 (61.1%) and 7.0 (33.6%).
N.D, no activity detected.
aND: no activity detected.
Maximum activities for the recombinant AmyA and GlaA in S. cerevisiae Y294 were observed at pH 4.0 and pH 4.5, respectively, with significant activity detected for both enzymes at pH 3 to 5.
The maximum activity of Mro APO was detected on day 24 of cultivation, i.e. 3 to 4 days after the pH had started to increase again reaching a maximum value of 7.5 on day 25.
The maximum activity of the purified recombinant meso-DAPDH was detected at 65°C in carbonate-KOH buffer at pH 10.5.
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