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Also, the maximum acetate accumulation was 9.93 mmol/L, which was used up at 120 h.
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a Maximal acetate accumulation and maximum specific growth rate measured in cultivations of xfpk expressing strains.
MGF-01 accumulated less than half the acetate that W3110red did and this lower acetate accumulation of MGF-01 may contribute to the more efficient glucose assimilation.
In a previous study, an Escherichia coli strain lacking the key enzymes (acetate kinase and phosphotransacetylase, ACK PTA) of the major acetate synthesis pathways reduced acetate accumulation.
Fig. 5 Phosphoketolase activity correlates with increased acetate accumulation and reduced growth rate and biomass formation.
One possible cause for the reduction of the fermentation efficiency is acetate accumulation.
b Acetate accumulation and optical density measurements during whole growth phase.
Reductions in feeding rate to limit acetate accumulation led in turn to increased product degradation.
Stable retention of an aceticlastic methanogen would contribute to efficient removal of acetate, thereby preventing acetate accumulation (Sasaki et al. 2007).
In order to reduce the acetate secretion during the fermentation fundamentally, a list of genes related to acetate accumulation in E. coli was selected and knocked out.
Expression of xfpk(LM) and xfpk CA) also led to increased acetate accumulation, albeit lower compared to xfpk(BB) expression.
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