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The mass range was 100 900 m/z, ICC target values were 8000 while the maximum accumulation time was set at 200 milli seconds.
Beside CV studies, other electrochemical studies were also performed to check the nature of peak current and peak potential and also to check the maximum accumulation time for both the amino acids.
The Ion Charge Control was 100000 and the maximum accumulation time was 200 ms. A total of 4 scans were averaged to obtain a MS spectrum and the rolling average was 2. For tandem MS experiments, the system was operated with automatic switching between MS and MS/MS modes.
A capillary voltage of 1700 1900 V (optimized on a per-chip basis) was used and the skimmer voltage was held at 30 V. Data were acquired at 25,000 m/z·sec−1 with a SmartTarget value of 500,000 and Maximum Accumulation Time of 200 ms. The MS acquisition range was from 300 to 1800 m/z.
The maximum accumulation time was set as 50 milliseconds.
The fragment intensity multiplier was set to 20 and maximum accumulation time was 2 s.
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Therefore, for 10−5 M l-serine and l-phenylalanine, the maximum accumulation times, 10 and 20 min, respectively, were employed.
The values of automatic gain control and the maximum accumulation times were 2 × 10 and 1,000 ms for orbitrap, and 3,000 and 120 ms for LTQ analyzer, respectively.
Maximum ion accumulation time was 500 ms for full MS scans and 100 ms for MS2 scans.
The maximum ion accumulation time was set to 100 ms for MS and 300 ms for MS/MS scans.
The maximum allowed accumulation time for CID was 200ms, the isolation width maintained at 2Da, activation q = 0.25, and activation time of 10ms.
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