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The absorbance of diluted dye solution was measured at the wavelength of maximum absorption using UV/Vis spectrophotometer.
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First of all, a phosphate buffer solution (PBS) of IBU (0.1 μg/mL) was prepared to find out the maximum absorption wavelength using a UV-visible spectrophotometer.
The decolorization potential was monitored by the decrease in maximum absorption of the dye using UV visible spectroscopy.
We anticipate that the concept of including the ZPVE into the calculation of the maximum absorption wavelength can be used also for other families of dyes to improve their predictability.
Maximum absorption intensity was obtained upon using 1 ± 0.5 mL of the buffer solution (Figure 4).
The photodegradation efficiency was monitored by measuring the absorbance of the centrifuged solutions at the maximum absorption wavelength of 664 nm using UV Vis spectroscopy (SHIMADZU, UV-2100) at room temperature.
In order to evaluate the efficiency of the degradation processes, the suspension was analyzed at a definite time interval, by recording variations at the maximum absorption around λ = 664 nm using a UV vis spectrophotometer (Shanghaijingmi instrument Co., Ltd. UV762).
This method depends on the reduction of purple DPPH radicals to a yellow coloured diphenyl-picrylhydrazine, and the remaining DPPH radicals which show maximum absorption at 517 nm were measured using UV-Vis spectrophotometer (Jenway 6405).
The ABTS* free radical scavenging ability model has the advantage of being more versatile due to the minimal spectral interference as the absorption maximum used is 760 nm, a wavelength not normally encountered with natural products [ 33].
Maps were plotted using the absorption spectra (maximum absorption is represented in red, and minimum absorption is represented in blue).
However, their maximum absorption at 630 nm limits the use due to e.g., tissue depth penetration.
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