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As with exonerate, the query sequence to be used is chosen from the profile alignment maximizing the sequence similarity to the predicted peptide sequence in the target.
2005], for the IM analysis, therefore, the data were pared down, firstly to remove any indel information not analysable by IM, while maximizing the sequence data (the basic IM dataset), and then subsampled to remove clearly recombinant regions, by sampling from the 5' region of each gene until a probable recombinant pattern is observed (reduced dataset 1).
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We choose the "optimal" set of 11 individuals at each locus in this way both to maximize the sequence contributions of individual loci to the inference of gene trees (maximizing n) and to maximize the potential for creating resolved gene trees (maximizing d).
In order to maximize the sequence length, some bases with a Phred score <20 were tolerated.
The initial multiple alignment of complete sequences was performed using PipeAlign [22] and manually adjusted to maximize the sequence conservation.
In order to maximize the sequence diversity, an additional paired-end Solexa sequencing (Illumina HiSeq2000) was employed.
After 454 pyrosequencing of I. indigotica transcription profiling, a paired-end Solexa sequencing was carried out to maximize the sequence diversity.
In addition, we have identified publicly available IMAGE clones that maximizes the 5' sequence to provide a full length construct when possible.
Therefore, finally we decided to analyze only first 188 bp of each read following the forward primer, which maximized the sequence yield while reducing the length of the analyzed fragment by only 5%.
Material collected more than 40 years ago was avoided in order to maximize the sequencing success rate.
S. chrysanthemifolius and S. aethnensis transcriptomes were multiplexed, and 100-bp paired-end reads were produced by running the multiplexed samples twice over two independent Illumina HiSeq 2000 runs to maximize the sequencing depth.
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