Similarly to most pattern detection methods in signal processing, the amount of data processed is reduced by first extracting candidate waveforms based on low-level detection analysis (by feature extraction), while subsequently classification is performed to maximize specificity of the overall method [3].
OCP ligation was performed with Ampligase for 20 min at 60°C, approximately 15°C above the Tm for the OCP 3' arm, in order to maximize specificity of ligation.
The use of RBT/CFT combinations, the most widely used serial scheme, is generally recommended to maximize specificity of the test result by ruling out false positive serological cross-reactions [ 11].
Obviously, maximizing specificity increases the number of false negative predictions, that is, a decreased sensitivity [ 31].
The resulting attenuation of odds ratios was substantially less for the "narrow" metric, further illustrating the importance of maximizing specificity when exposure prevalence is low.
In our study, maximizing specificity was desirable because infection of I. ricinus with B. microti was a priori assumed to be rare, and underestimation of prevalence therefore is conservative.
Although the number of patients in the transudate group was very small (n = 5), both GAGR and pGAG correctly detected them all, maximizing specificity, while pGAG misclassified 6 out of the 45 exudates.
This low recall rate may be due to the stringent threshold of Cufflinks, which aims at maximizing specificity.
Separation of this region was specifically chosen to maximize specificity at the expense of sensitivity.
Details of the variant calling pipeline are given in Sergouniotis et al. (2014) and Supplementary Method 5. Aiming to estimate the burden of mutations at genome-wide level, we chose thresholds for variant QC metrics to maximize specificity over sensitivity, accepting loss of power to detect a significant association in favour of a reduced type I error rate (Supplementary Method 6.1).
