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In these applications, the primary goal is that of optimizing probe sets and hybridization conditions to maximize the specificity of species-specific probe/target interactions, possibly at the expense of decreased assay sensitivity and thus the majority of microarrays used for species identification are oligonucleotide-based.
We optimized methods to reduce background and maximize the specificity of staining.
In an effort to maximize the specificity of the NBQX effects, we first tested a range of concentrations (250 nM, 500 nM, 750 nM, 1 µM, 2 µM) to determine the lowest effective dose that disrupted directional responses in the DSGC.
We did this to maximize the specificity of our codes for diabetic foot infections.
These values have been chosen to maximize the specificity of each test, though at the cost of sensitivity.
A basic cut-off of optical density of 20% was used to maximize the specificity of the ELISA.
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The lightweight IDS has been developed by using a wrapper based feature selection algorithm that maximizes the specificity and sensitivity of the IDS as well as by employing a neural ensemble decision tree iterative procedure to evolve optimal features.
The lightweight IDS has been developed using a wrapper-based feature selection algorithm that maximizes the specificity and sensitivity of the IDS as well as by employing a neural ensemble decision tree iterative procedure to evolve optimal features.
The models were developed using a threshold providing at least 90% sensitivity, and maximizing the specificity, assessed using leave one pair out cross-validation (LOPOCV).
Classification performances were assessed by leave one pair out cross-validation with a threshold that resulted in ≥90% sensitivity and maximized the specificity.
Histidine microswitches that rectify DNA protein interactions should be of practical use for maximizing the specificity of targeting, for instance, in design of genome editing nucleases.
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