It follows from (12) that GPavg becomes maximum when for given (C S I,C S I PU) the transmission parameters (ϕ,E) maximize the expression between brackets in the second line of (12), under the constraints (2) and (4).
These synthetic DNAs were designed according to the typical codon usage of E. coli to maximize the expression.
Unc45bFlag expression in cultured muscle cells does not disrupt differentiation or the assembly of the muscle specific cytoskeleton and therefore, the adenovirus infected cells could be maintained for 4 5 days to maximize the expression and recovery of the recombinant protein.
To maximize the expression of vIL-10 we generated a tetracycline-inducible CaMV 35 S promoter including an enhancer sequence.
Voltage applied at 40 V·cm-1 was found to maximize the expression of reporter gene with 6 pulses (Fig. 1B).
Promoter characteristic studies are required before they can be utilized to maximize the expression of targeted genes in desired conditions.
Then, for class 2 data (nonsynonymous sites), these parameters (τ and υ) are fixed in order to maximize the expression and estimate the selection parameter, γ.
Embryos were collected for 20 24 h and then transferred to a water-bath at 30°C to maximize the expression of the transgene.
(E-Step) Based on the parameters predicted Θ the expectation of the log-likelihood is calculated as follows: (1) P V ∣ D, Θ t = P U, D, Θ t ∑ D ∈ D n P U, D, Θ t. (M-step) The new parameters are calculated to maximize the expression according to the expected log-likelihood found on the E-Step.
(1) (E-Step) Based on the parameters predicted Θ the expectation of the log-likelihood is calculated as follows: (1) P V ∣ D, Θ t = P U, D, Θ t ∑ D ∈ D n P U, D, Θ t. (2) (M-step) The new parameters are calculated to maximize the expression according to the expected log-likelihood found on the E-Step.
