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Translation of ultrasound molecular imaging to the clinical arena will require optimization of contrast agent design to maximize specific binding, and customization of imaging systems to sensitively detect the binding events.
8 Our aim was to design and develop a probe for the BRD7 and BRD9 bromodomains, achieving potency and selectivity with a suitably decorated fragment 9 rich in sp-hybridized carbon atoms that, guided by biophysical assays, was designed to maximize specific binding interactions whilst retaining synthetic tractability.
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PEEK tubing was used for all fluid paths involving [18F]fluoride to maximize specific activity [23].
For other applications, a more structured approach to maximize specific multivalent effects is best for achieving the desired interaction.
However, non-specific binding residues can help specific binding residues to increase binding specificity as well.
specific binding ratio.
Concentration of specific binding sites.
The specific binding was then obtained as the total binding minus the non-specific binding.
Place two 96-well plates for specific binding and non-specific binding respectively.
Specific binding of 125I-[Sar1 125I-[Sar1Ile8]Angalculated as total (non-AT2R) mIIus non-specific binding.
Robust specific binding was observed in all four brain regions tested with cortex showing the highest specific binding with a specific/non-specific binding ratio of ~18.
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