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In the absence of a reference genome, we generated a de novo assembly using a methodology designed to maximize reference coverage while minimizing redundancy and chimera rate [ 29].
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Reference Coverage.
Average reference coverage is 500.8-fold 500.8-fold
This gave a reference coverage of around 1000×.
(B) Histogram of reference coverage across the assembled contig.
De novo assembly was conducted for the individual libraries and for the four libraries pooled to maximize the coverage and to provide a more comprehensive reference transcript.
We have not used this option, again to maximize the coverage of the extracted variants with respect to the reference set.
You should try to find a time where you can maximize your coverage.
But the report also shows that the senior officials paid significant attention to timing the case to maximize media coverage.
This was done to maximize the coverage and use all available IGS stations.
Their model aimed to maximize the coverage and minimize total costs.
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