Sentence examples for maximize gene silencing from inspiring English sources

Furthermore, the structure activity relationships that favor cell internalization and maximize gene silencing, while maintaining cell type-specific penetration have yet to be determined.

Here, we performed analyses to maximize gene sampling.

These vectors can be used to simultaneously knock down the expression of different genes or to maximize the gene silencing of a single gene.

Conditions for multiple heat shocks were determined empirically for each genotype to maximize target-gene silencing (determined by western blot) without causing lethality.

To determine whether our dual shRNA expression vector can be used to maximize the efficiency of gene silencing we targeted two different regions in exon 1 of p16INK4b mRNA.

We next determined whether these vectors can be used to maximize the efficiency of gene silencing by producing siRNA targeting different regions of a gene or by producing more copies of a single siRNA targeting the same region of a gene.

As shown in Fig. 3b, the production of Si6 by two siRNA cassettes (Vec66) led to a more profound inhibitory effect on TRAF6 expression than single siRNA cassette (Fig. 3b), indicating that the vectors containing multiple siRNA cassettes may also be able to maximize the efficiency of gene silencing by expressing more copies of siRNA targeting the same region of a gene.

The vectors containing multiple siRNA expression cassettes can serve as useful tools for maximizing the efficiency of gene silencing.

The vector-based siRNA approach has several advantages: first, a selection marker (i.e. an antibiotic resistance gene) may be included in the vector for subsequent selection of positively transfected cells to maximize the effect of the gene silencing.

With the availability of completely sequenced genomes, siRNA or vector-based shRNA libraries can be constructed with specific designs to maximize the probability of potent target gene silencing and to minimize the risk of off-target effects (Huesken et al., 2005; Turner et al., 2008).

In all experiments, an Agrobacterium strain harbouring a gene encoding the TBSV P19 protein was added to maximize protein production by suppression of gene silencing [34].