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Many viruses have evolved to maximize expression of their proteins in host cells and are therefore a good starting point for construction of efficient expression vectors.
The promoter sequence of the thiolase gene was chosen in order to maximize expression of the adh gene, since the thiolase gene of C. acetobutylicum was reported to be constitutively expressed (Hartmanis and Gatenbeck1984; Tummala et al.1999; Alsaker and Papoutsakis2005).
Thus, various cultivation conditions might be employed to differentially maximize expression of certain NKLRs of interest.
Thus, bacteria have been grown with high concentrations of CO to maximize expression of heterologous globins (62) and CO is much less effective than CORMs in inhibiting bacterial growth (52) and respiration (74).
In order to maximize expression of the cold-active β-galactosidase in Halobacterium sp. NRC-1, we introduced a cold-active promoter for the cold shock protein gene, cspD2, into a haloarchaeal expression vector [ 40].
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Crosses for the LOF screen were performed at 22 23°C, whereas crosses for the overexpression screen were performed at 25°C in order to maximize expression under control of the GMR-GAL4 transgene.
If selection is acting on gene sequences to maximize expression efficiency, we might expect to find a relationship between gene expression profiles and some descriptions of sequence parameters.
The results suggest that 570 μM and 60 μM are the optimal IKVAV concentrations for 2D and 3D neural differentiation, respectively, to maximize mRNA expression of neuron-specific markers and neurite extension while minimizing apoptotic activities in cultured cells compared to those exposed to higher IKVAV concentrations.
To maximize the expression of vIL-10 we generated a tetracycline-inducible CaMV 35 S promoter including an enhancer sequence.
Voltage applied at 40 V·cm-1 was found to maximize the expression of reporter gene with 6 pulses (Fig. 1B).
Promoter characteristic studies are required before they can be utilized to maximize the expression of targeted genes in desired conditions.
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