Sentence examples for maximize expression in from inspiring English sources
Plasmid DNA was purified using the NucleoBond Xtra Midi kit (Macherey-Nagel), followed by an isopropanol precipitation and resuspension of the DNA pellet in ultrapure H2O to prepare highly concentrated plasmid stocks and maximize expression in TX-TL reactions.
The sequences of the synthetic genes are based on naturally occurring polyketide synthase genes but they are redesigned by custom‐made software to optimize codon usage to maximize expression in E. coli and to provide a standard set of restriction sites to allow combinatorial assembly into unnatural enzymes.
The second observation was the apparent lack of improvement, in terms of relative brightness, exhibited by mCitrine and mCherry when compared, respectively, to F-YFP and Mc-mCherry despite the codon optimization undertaken specifically to maximize expression in vivo.
One of the major problems in estimating to what extent a specific gene feature has undergone selection to maximize expression efficiency in humans is the wide variation that exists in RNA and protein levels among distinct tissues and even within the same tissue in different physiological and pathological conditions.
Many viruses have evolved to maximize expression of their proteins in host cells and are therefore a good starting point for construction of efficient expression vectors.
In order to maximize expression of the cold-active β-galactosidase in Halobacterium sp. NRC-1, we introduced a cold-active promoter for the cold shock protein gene, cspD2, into a haloarchaeal expression vector [ 40].
The promoter sequence of the thiolase gene was chosen in order to maximize expression of the adh gene, since the thiolase gene of C. acetobutylicum was reported to be constitutively expressed (Hartmanis and Gatenbeck1984; Tummala et al.1999; Alsaker and Papoutsakis2005).
Crosses for the LOF screen were performed at 22 23°C, whereas crosses for the overexpression screen were performed at 25°C in order to maximize expression under control of the GMR-GAL4 transgene.
Selective pressure to maximize expression rate and minimize energy expenditure could be dominant in highly expressed genes as originally suggested [ 156].
The similar transgene expression in mt-1 and ppk transgenic bacteria indicate that it is possible to express prokaryotic and mammalian genes effectively in bacteria if the vector is engineered with proper regulatory elements to maximize expression.
GGS-selected genes, which maximize expression information about unmeasured genes, can be combined with candidate gene sets as a cost effective way to increase the amount of gene expression information obtained in large studies.
