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Selective pressure to maximize expression rate and minimize energy expenditure could be dominant in highly expressed genes as originally suggested [ 156].
For each index, we calculate the optimal threshold that would maximize expression (5).
Many viruses have evolved to maximize expression of their proteins in host cells and are therefore a good starting point for construction of efficient expression vectors.
The promoter sequence of the thiolase gene was chosen in order to maximize expression of the adh gene, since the thiolase gene of C. acetobutylicum was reported to be constitutively expressed (Hartmanis and Gatenbeck1984; Tummala et al.1999; Alsaker and Papoutsakis2005).
The sequences of the synthetic genes are based on naturally occurring polyketide synthase genes but they are redesigned by custom‐made software to optimize codon usage to maximize expression in E. coli and to provide a standard set of restriction sites to allow combinatorial assembly into unnatural enzymes.
Thus, various cultivation conditions might be employed to differentially maximize expression of certain NKLRs of interest.
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Thus, the Baum-Welch algorithm searches for the parameters Θ that maximizes Expression (1).
In the present research, we attempted to develop an exact protocol to maximize gene expression while minimizing expression variability and tissue damage.
We optimized the transfection and cell culture conditions to maximize protein expression in a 96-well format so that the expression levels were comparable with the levels observed in shake flasks.
In experiments carried out with rat, T-cells, CD28, and TCR stimulation could upregulate more RANKL expression than TCR or CD28 stimulation alone, indicating that costimulatory signals are necessary to maximize RANKL expression.
In December, the State Department asked for financing proposals from organizations with technologies that "maximize free expression and the free flow of information and increase access to the Internet".
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