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Typically, we balance the third-order spherical aberration C3 with the fifth-order spherical aberration C5 to maximize contrast transfer close to 1 [36] and capture all elastically scattered electrons.
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The fluorescent images were post-processed in Photoshop to maximize contrast.
Color scales were monotonically non-linearly adjusted in each case to maximize contrast.
A high multiple of threshold was used in order to maximize contrast and BOLD (blood oxygen level-dependent) signal strength.
Lighting consisted of a matrix of LEDs that emitted cool white light positioned to maximize contrast and reduce reflection.
They can be loaded with separate core and surface payloads, maximizing contrast for two imaging methods.
We assigned pseudocolors to each channel to make merged images more comprehensible by maximizing contrast using AxioVision software (Carl Zeiss).
Contrast transfer function.
Align tilt series and correct contrast transfer function (CTF).
Quantification of contrast leakage to produce measures of contrast transfer coefficient (Ktrans) is more difficult[ 23 ].
Therefore, it is desired to maximize the multiplication of effectiveness and capacity ratio to maximize heat transfer.
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