Sentence examples for maximize cell breakage from inspiring English sources
The RNA isolation followed standard procedures using Trizol reagent (Invitrogen, Germany), however modified to include a homogenization step with 0.5 mm glass beads to maximize cell breakage.
Cell breakage was reduced considerably for cells grown in medium supplemented with PF-68.
Higher cavitation yield causes more acoustic cavitation in the medium, which then causes floc disintegration and cell breakage, leading to release of intracellular materials to the aqueous phase.
Cell breakage was corroborated by light microscopy.
Cell breakage efficiency was assessed using microscopic tests.
This latter study used freezing/thawing cycles combined with ultrasonic treatment for cell breakage.
In general, combinations of a variety of physical and chemical methods are exploited for cell breakage.
Cell breakage buffers were supplemented with an EDTA-free protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN).
Heating caused a disruption of cell arrangement and cell breakage as a result of the thermal destabilization of pectin materials.
The high amount of soluble FtsY observed after cell fractionation is most likely to be an artefact of cell breakage.
In fact, pentameric HoxEFUYH hydrogenases tend to dissociate following cell breakage into the diaphorase (Hox-EFU) and hydrogenase (HoxYH) subunits.
