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After further purification and fusion tag removal, the maximal yield was 50 75 mg per L of expression media.
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The maximal yield is reached approximately 20 h after the start of the reaction.
The optimal levels of each factor were obtained from the value of k i A, and these results lead to the conclusion that the factors that provided the maximal yield were a reaction temperature of 60°C, a solvent quantity of 35 mL, a reaction time of 2 hrs, and an amount of catalyst of 1.5% (based on the lipid weight).
The S/H ratios were approximately 1.0 with both methyl and ethyl esters, when the maximal yields were achieved.
By flux analysis, the pathway mode leading to maximal production yield was simplified with identification of key reaction steps.
It was shown that optimum selectivity with the maximal isomer yield was obtained for the catalyst where calcination and Pt acid decomposition were hold simultaneously at high temperature.
The moisture content of the spray-drying powder of A. cearensis varied from 3.72 to 5.85% (w/w), while the maximal process yield was 41.1% (w/w).
The synthesis started typically with 26 28 GBq of the dried K[18F]F/K222 complex and was finished within 60 75 min and maximal 18F incorporation yield was 6%.
Maximal conidial yield was recorded for rice at a pH of 7.01; at 7.06 for sorghum and at 6.76 for barley.
The maximal ethanol yield was 0.411 g g−1 WHB when P. stipitis was used along with T. reesei which is positively correlated to the theoretical yield 0.429 g on the basis of biomass.
Prolonged treatment for 24 h with a 50 50 mixture of Celluclast 1.5 L and Ultraflo L at 50 °C, pH 5 exhibited a synergistic effect in xylose release and 62 wt.% of the theoretically maximal xylose yield was achieved.
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