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Already after 96 h of cultivation, the engineered production strain BW86 reached up to 1 g cyanophycin per liter culture, approximately a 4-fold increase over the previously reported maximal yield obtained after 12 days of cultivation.
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Quantum yield was calculated by the equation F'ms-Fs/F'ms according to Genty [ 63], where Fs is the steady state fluorescence under environmental conditions, and F'ms is the maximal fluorescence yield obtained after a saturating light pulse.
Although the maximal H2 yield obtained in this experiment represents less than 60% of the maximum theoretical yield that can be obtained by dark fermentation (4 mol H2/ mol glucose), only a few studies have reported higher yields, sometimes using much more sophisticated and expensive systems [ 39].
The yield obtained was 40%.
The yield obtained was 89%.
The yield obtained was 5.1% (w/w).
The percentage of yield obtained as 40.81%.
The FFAs yield of 6.1% is higher than the maximal yield of 4.8% obtained by Lu et al., but is still much lower than the theoretical maximal yield of ~30% (purely stoichiometric yield) [ 15].
Evaluation of photoheterotrophic H2 production using iRsp1095 indicated that maximal yield would be obtained from growing cells, with this predicted maximum ~50% higher than that observed experimentally from wild type cells.
maximal and variable fluorescence yields obtained from a dark-adapted sample upon application of a saturation pulse of radiation, respectively.
When grown on d-glucose, the yields obtained with R. oryzae are close to this maximal yield.
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