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The yeast genes described in large-scale phenotypic analysis as being required for maximal yeast tolerance to ethanol [ 15], high glucose concentrations (as those found in industrial growth media) [ 16], acetic acid [ 17], vanillin [ 18] and furfural [ 19] were compared.
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Yeast tolerance to lignocellulosic hydrolysate inhibitors has also been improved by genetic engineering strategies (Table 1) [40].
A better understanding of molecular mechanisms underlying yeast tolerance to these fermentation-associated stresses is essential for improvement of yeast stress tolerance by genetic engineering approaches.
Mechanisms of yeast tolerance at the genome level remain unknown.
A non-recombinant way to increase yeast tolerance to hydrolysates is by encapsulation of the yeast.
However, the MNN10 gene may be essential to increase yeast tolerance of ethanol-induced stress [ 61].
An agar plate assay was used to indirectly determine the yeast promoter activity as the maximal Zeocin tolerance levels of the P. pastoris host cells.
On average, patients had a limited maximal exercise tolerance.
The maximal Zeocin tolerance level determinations were carried out with minimum four technical recurrences.
The incremental CPET quantifies VO2peak, which is considered the gold standard for determining maximal exercise tolerance.
Maximal exercise tolerance (VO2peak and RPmax) and submaximal exercise tolerance (VO2AT and 6MWT), FEV1 and FCV were correlated with eGFR (Table 4 and Figure 2).
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