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Sorghum straw, an inexpensive and abundant source of carbon supported maximal xylanase activity (11,855 units/g dry substrate).
The maximal xylanase activity towards oat spelts xylan occurred at pH 6.0, and xylanase activity decreased rapidly by the pH increasing, indicating that Xyn10A is an acidic xylanase (Fig. 2c).
In shaking condition), maximal xylanase activity was observed on 3.5-day-old cultures, corresponding to the values of 25.85 U/mL.
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Under optimized condition maximum xylanase activity found 260 U/ml.
Notably, it showed significantly higher xylanase activity (2500 5023 mU maximal value) than numerous fungus isolates of various herbivore origins (Cao and Yang 2011; Sirohi et al. 2013; Thareja et al. 2006; Tripathi et al. 2007).
specific xylanase activity per milligram of protein.
The xylanase activity was determined according to Biely (Biely 1997).
Xylanase activity was found to decrease sharply with prolonged incubation.
These enzymes exhibited optimal xylanase activity at pH 4.0 – 4.0.
The residual xylanase activity was then determined at pH 5.2.
Clear bands against a dark background indicated xylanase activity.
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