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Kinesin motors produce a maximum of ~5 pN of force for every 8 nm step they take along the microtubule., Thus, we can estimate that in our assay, kinesin motors can do maximal work on the order of ~10 kBT.
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Initial walking intensity will be prescribed at 75% of the maximal work rate achieved on the ISWT while cycling intensity will be based on data obtained from the 6-minute walk test (6MWT) [ 27].
Cavalheri V, Hernandes NA, Camillo CA, Probst VS, Ramos D, Pitta F. Estimation of maximal work rate based on the 6-minute walk test and fat-free mass in chronic obstructive pulmonary disease.
Trappenburg et al. [ 24] focused on maximal work rate, functional exercise capacity, and quality of life as dependent variables.
It is based on parallelism, maximal work by each datum, reduction of unnecessary data access, sorting, and data compression.
More than one century later, a force-velocity relationship was deduced from experimental studies on the relationship between maximal work and contraction time in man.
Prior to the experiment, subjects were accustomed to exercise on the knee extension ergometer and maximal work capacity (Wmax) was determined for each leg as described by Andersen and Saltin (1985).
Work was normalized to individual maximal work capacity (Wmax).
The topics of the first studies on muscle properties were not related to mechanics (force, velocity, power) but energetics (maximal work, efficiency of muscular work, metabolism) for the isolated muscle as well as man.
During the preliminary trials, participants were familiarized with the methodology before completing incremental exercise on a semirecumbent cycle ergometer (Lode Angio, Groningen, The Netherlands), with a backrest inclination of 45°, to establish the maximal work rate, maximal heart rate (HR), and V̇ o2 max.
In healthy individuals, most activities require only a fraction of maximal working capacity, as assessed by maximal VO2 (VO2 Max).
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