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A stimulus intensity that evoked synaptic responses that were 60 to 75% of maximal was determined for each animal (400 to 800 μA; mean, 576 μA).
For this approach, the fraction of total reaction products due specifically to nucleotide addition at the completion of the reaction (i.e., when [ES] is maximal) was determined from the maximal amplitude of the N 2 addition reaction (Padd,max) divided by the total reaction amplitude (Ptotal).
Similar(58)
p50 value – the partial oxygen pressure (pO2), at which the cellular respiratory rate is half-maximal – was determined as before [28].
In our study, the maximal value was determined excluding such foci, but this process may be somewhat difficult in patients with very heterogeneous lung disease.
In sedated patients, after the warm-up, the intensity that produced a maximal response was determined, and the 60% of that value was used as the starting point.
The contractile function of each segment and a maximal contraction was determined with 10 ìM phenylephrine (Phe).
For an anchor gene its maximal clique was determined.
After four measurements of basal respiration, CCCP was injected (12 μM) and maximal respiration was determined.
Maximal respiration was determined after stimulation with 0.8 μM FCCP, an electron transport chain uncoupler.
Spontaneous and maximal release was determined in the presence of medium alone or of 1%NP-40NP-40
Lesion maximal thickness was determined by measuring from the skin surface to the deepest point of the posterior margin.
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