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This is consistent with experimental observations [ 30, 31] on S. cerevisiae cells, whose volume indeed increase steadily until they reach a relatively constant maximal volume and enter senescence.
Overall, INT performed at intensities 33 and exercise-to-rest ratios 34 that elicit maximal volume and pressure overloads on the myocardium and VO2 responses near maximal oxygen uptake (VO2max) are quite likely optimal in terms of cardiac output, blood flow, shear stress, recruitment and increased oxidative capacity of fast twitch (FT) fibres.
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At the time points of assessment, supernatants were equilibrated to the maximal volumes and served as a source to immunoprecipitate EpEX with specific antibodies.
APE was added to the culture medium in a maximal volume (<0.4%) and the same volume of ethanol as the solvent was added to the medium of the control cells.
The VOI was chosen to include a maximal volume of the tumour, and thus varied with tumour size between approximately 0.2 and 6 cm.
The GPG preceded global minimum temperatures and maximal volume of ice, which occurred in the last ~ 800 kyr, as recorded in the marine δ18O record.
As expected, tumors derived from metastatic WM1617 cells grew rapidly and reached maximal volume allowed between 5-11 weeks post-injection.
Therefore we tried to avoid concentration and searched for a balance between the minimal volume for depolymerization and the maximal volume for the second polymerization.
The parameter derived from the PV loop was the maximal volume difference between the inspiratory and the expiratory limbs of the PV loop at a given pressure, indicating the maximal hysteresis area.
During the third month post-operation, the balloon insufflation was kept with the maximal volume for 4 hours each time and 4 times per day.
Such association was much less strong (quantitatively and qualitatively) for LA maximal volume (data not shown).
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