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In the backpressure-voltage experiment, along with the backpressure changing from 0 to the maximum of 7.1 kPa at an excitation voltage of 210 Vp p, the sensing voltage also changes from 0 to the maximal voltage of 16.6 Vp p correspondingly at a fixed frequency of 30 Hz.
A constant current stimulator (Digitimer DS7A, Hertfordshire, United Kingdom) was used to deliver a square-wave stimulus of 1000 µs duration with maximal voltage of 400 V.
IEF was performed at a maximal voltage of 500 V for 600 voltage hours.
IEF was performed at a maximal voltage of 500 V for 600 voltage hours or 8 000 voltage hours.
A high-voltage stimulator (Digitimer DS7AH, Digitimer, Hertfordshire, England) was used to deliver a square-wave stimulus of 0.2 ms duration with a maximal voltage of 400 V.
A high-voltage stimulator (Digitimer DS7AH, Digitimer, Hertfordshire, England) was used to deliver the doublet (square-wave stimuli of 0.2 ms duration) with a maximal voltage of 400 V.
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Activation curves, determined from peak tail current normalized to the maximal value and plotted against the test pulse voltage, were fitted with Boltzmann equation (y=[1+exp{(V− V1/2)/ k}]−1), where V1/2 is the half-maximal voltage of activation, and k is the slope factor.
Criteria for artifact rejection within an epoch were a maximal voltage step of 50 µV, a maximal difference between two values in a segment of 100 µV, and a minimal and maximal amplitude of −100 µV and 100 µV, respectively.
Only trials with correct responses and without artifacts – defined as any signal exceeding ±60 µV, bursts of electromyographic activity (permitted maximal voltage steps/sampling point of 50 µV), and activity lower than 0.5 µV within intervals of 500 ms (indicating dead channels) – were selected on an individual-channel basis, prior to averaging.
The value of the maximal voltage obtained in the registration of maximal clenching was analyzed.
By perfusion with 100 nM martentoxin, the activation curve of gBK channels as well as the half-maximal voltage (V1/2) of activation was not significantly shifted with the application of 100 nM martentoxin (99.5±2.94 compared to 96.3±2.64,n = 5, p>0.05) (Fig. 4D and Table 1).
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