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The obtained maximal voltage value (600 mV) has also been compared to literature data.
In the backpressure-voltage experiment, along with the backpressure changing from 0 to the maximum of 7.1 kPa at an excitation voltage of 210 Vp p, the sensing voltage also changes from 0 to the maximal voltage of 16.6 Vp p correspondingly at a fixed frequency of 30 Hz.
At 800 °C and a KOH/MEGO mass ratio of 6.5, a maximum specific surface area of 3100 m2/g was obtained and a high specific capacitance of 172 F/g (at 1 A/g constant current and 3.5 V maximal voltage) was measured in a two-electrode cell with a-MEGO electrodes in an organic electrolyte.
Thus, the maximal voltage is defined as the threshold voltage.
Table 3 summarises the main result of our experiments, indicating the total wall clock time, the average power level, and the total energy consumption of each benchmark under three different energy policies: dynamic voltage and frequency scaling (DVFS) as described in Section 5, dynamic frequency scaling at maximal voltage (DFS), and maximal voltage and frequency settings (MVF).
A constant current stimulator (Digitimer DS7A, Hertfordshire, United Kingdom) was used to deliver a square-wave stimulus of 1000 µs duration with maximal voltage of 400 V.
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The parameters for fast inactivation were characterized by the half-maximal voltage Vf and the slope factor k f; correspondingly Vs and k s for slow inactivation.
The activation curve of α+β1 BK channels and the half-maximal voltage (V1/2) of activation were also insignificantly moved (65.5±1.68 compared to 68.5±3.03, n = 8, p>0.05) (Fig. 5D and Table 1).
Data were fitted to the Boltzmann equation GNa = [1+exp V1/2−V)/k]−1 where V1/2 is the half-maximal voltage, k is the slope factor, and the Na+ conductance GNa is defined by GNa = INa,norm/(V−Vrev).
By perfusion with 100 nM martentoxin, the activation curve of gBK channels as well as the half-maximal voltage (V1/2) of activation was not significantly shifted with the application of 100 nM martentoxin (99.5±2.94 compared to 96.3±2.64,n = 5, p>0.05) (Fig. 4D and Table 1).
Importantly, while IKr densities were affected by the mutation, voltage dependence of activation parameters (half-maximal voltage (V1/2) and slope factor (k)) were similar, both in mutated and corrected LQT2-hiPSC-derived CMs and in wild-type and mutated hESC-derived CMs.
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