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Infection of T47D cells with Ad5AMUCH_RSV-NIS at MOI 20 showed maximal uptake at 24 hours after infection, followed by a twofold decrease in I uptake at later time points.
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In the present study, we administered an activity of 400 MBq/kg body weight, which results in mean absorbed dose rates of approximately 0.2 to 0.6 Gy/h at the maximal uptake, 24 h p.i. [30].
We suppose that glucose uptake follows Michaelis Menten kinetics, so that the uptake rate is given by V max ψ / (ψ + K ), where ψ is the concentration of glucose, Vmax is the maximal uptake rate, and K is the concentration at which the uptake rate reaches half of its maximum.
Although guidelines serve an important purpose in clarifying best practice and reach a wide audience at little cost, they are insufficient to produce maximal uptake.
The uptake by the synaptosomes at 30 min in [H]dopamine only was taken as maximal uptake (100%).
To evaluate insulin-induced changes in DAT-mediated DA uptake, the initial portion of the falling phase of evoked [DA]o curves was fitted to the Michaelis Menten equation to extract Vmax (maximal uptake rate constant)56.
Kinetic analysis of the developing vesicular uptake mechanism revealed no change in Km, while maximal uptake increased progressively from birth to maturity.
In vitro uptake studies using optical and computed tomography imaging in the RAW 264.7 macrophage cell line identified the formulation that promoted maximal uptake.
Therefore, inflammation prevents maximal uptake of oxygen by cells.
In the second PET scan, the region of interest will be placed at the same position as in the baseline PET as a reference of initial maximal uptake.
Arterial oxyhaemoglobin saturation (SaO2) is strongly linked to the ability to maintain maximal oxygen uptake at altitude.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com