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Transduction by TAT-Y13 was observed within 25 min after protein addition to the culture medium reaching maximal transduction after 90 min (Fig. 2A).
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The neutralizing antibody factors (NAF) were defined as the reciprocal plasma dilution inhibiting maximal transduction by 50%.
The percentage of transduction inhibition is then calculated for each plasma dilution relatively to the maximal transduction efficiency which is determined by a control AAV infection in presence of PBS.
As shown in Figure 2A, both integrative and nonintegrative lentiviral vectors were capable of transducing FL-DC with maximal transduction of efficiency of 60% and 56% respectively.
Dual AAV minigene delivery has resulted in saturated transduction following direct muscle injection and widespread transduction after intravenous injection in mouse models of DMD.
The shorter path length implies that the edges are selected for maximal signal transduction integrity.
The level of enablement may be maximal immediately after consultation.
DPPB had its maximal effect after 24 h of incubation.
Compared to pharmacokinetic properties, pharmacodynamic properties are more difficult to scale [ 156], since they are not often related to bodyweight (e.g. receptor occupancy, transduction, maximal effect, etc).
The slice preparation started maximal 10 min after the tissue resection.
In this study, the response was maximal after 30 min, and then actin patches repolarized.
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