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For combined applications, the tumor cells were stored at 37 °C for 4 h between RT and/or CT and HT treatment, as it is the maximal time frame between the treatments in clinical application.
Previous studies in our lab showed that after a 6-h period, problems may arise due to the long fasting period, during which subjects remain at rest and, hence, this was considered as the maximal time frame for the chosen experimental set-up.
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The maximal planning time frame is limited to 40 days.
Incubation of these nondividing EpH4 cells with Dex resulted in a 4-6-fold reduction of AQP5 expression at the mRNA and protein level measured at different time points of a maximal 72-hour time frame).
We thus focused on Sorafenib-mediated effects 48 and 72 h post EGF/insulin treatment as this is apparently the time frame of maximal DNA replication in mitogen-stimulated hepatocytes.
Understanding the pattern of change would help clinicians to communicate the expected results of surgery and time frame for reaching maximal recovery to their patients.
Participants provided information on television (TV) viewing and car time in 1982 and completed a maximal exercise test during a 1-year time frame; they were then followed until mortality or through 2010.
Overall, this study underscores that stem cell therapy at optimal time frame is crucial to obtain maximal therapeutic effects that can restore functional deficits or stop the progression of AD.
The US was delivered at unpredictable time points within the time frame of the CS± after the maximal spectrum of the colour was achieved to maximize unpredictability (Grillon et al., 2006).
This time frame coincided with the acquisition of maximum rolling efficiency and maximal expression of CCR-2 and LFA-1 by monocytes.
Rab7 expression was maximal 16 24 h post-transfection and experiments were conducted during this time frame.
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