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This constraint reduces the yield and renders the maximal theoretical yield too loose to be predictive.
Replacing sulfate by thiosulfate or sulfide increased the maximal theoretical yield in C. glutamicum up to 0.68 (C-mol) (C-mol)−1.
For a desired product, the framework predicts the theoretical yield of the product, and generates all the possible reaction network topologies capable of producing the maximal theoretical yield.
This allowed calculation of the maximal theoretical yield of L-galactonic acid that can be obtained from CPW (1 mol of L-galactonic acid per 1 mol of D-galacturonic acid).
For example, in the case of glycerol, allowing up to 10 concurrent gene knockouts has led to a production yield of only 66% of the maximal theoretical yield, compared to 89% achieved by directly using a biosensor for glycerol (see Table S2 and S3 for details).
The maximal theoretical yield of P3HB from glucose is 0.48 g P3HB/g glucose.
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Although the maximal H2 yield obtained in this experiment represents less than 60% of the maximum theoretical yield that can be obtained by dark fermentation (4 mol H2/ mol glucose), only a few studies have reported higher yields, sometimes using much more sophisticated and expensive systems [ 39].
OptKnock-proxy's predictions reflect the maximal theoretical production yield of a chemical of interest, assuming a maximal secretion rate of the proxy metabolite.
FBA is applied to predict the maximal theoretical production yield of a chemical of interest, while enforcing a minimal growth rate of 0.1 mmol/gr(DW h to maintain viability (the same threshold used in the implementation of OptKnock and RobustKnock; other choices of this threshold gave quantitatively similar results).
By using a maximal theoretical ethanol yield of 0.51 g g-1 (yield for hexoses) the percentage of the theoretical yield was defined as Y*SE = 100*YSE/0.51.
Ethanol yield in SHF and SSF was calculated based on the maximal theoretical ethanol yield from consumed glucose only, which is 0.51 g ethanol/g glucose.
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