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Maximal tetanic force (Po) generation was typically achieved at 200 Hz.
Absolute maximal tetanic force production (Fmax) at 4 and 8 weeks is shown in Figure 2, middle panel.
To evaluate whether histological differences between the regenerated muscles from control and mutant mice were associated with a difference in the functional recovery from injury, maximal tetanic force was measured before and 21 days after CTX (Fig. 5D).
In order to evaluate the possibility that abnormalities in neuromuscular transmission were contributing to the maximal tetanic force deficit, we evaluated force-frequency curves from wild type and KN1 control males.
To investigate whether deficits in force production in male KN1 mice could be accounted for by decreased TA mass, we calculated specific force (sPo), the maximal tetanic force output normalized for the cross sectional area of the TA muscle.
In comparison, the muscles from Sham rats displayed a 50% increase in maximal tetanic force production from 4 to 8 weeks, likely as a result of their dramatic increase in their type II fiber cross sectional area.
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nNOS-deficiency caused reductions in skeletal muscle bulk and maximum tetanic force production in male mice only.
Tetani were analysed for maximum tetanic force.
The fibre length was adjusted to obtain maximum tetanic force.
CMV alone decreased maximum tetanic force (Po) production by 35% versus control (P < 0.01).
Maximal tetanic forces (P0) of the extraocular muscles were only ~25% of the P0 generated by EDL bundles.
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